The Institut Pasteur has a long history of implementing a One Health approach to address emerging infectious diseases. It has worked jointly with animal and human health sectors on diseases such as rabies, H5N1, and MERS-CoV through activities like field investigations, laboratory training, and vaccine development. While One Health approaches have been adopted in policies, local implementation remains a challenge due to issues like limited resources, sectoral barriers, and communication difficulties. The Institut Pasteur aims to strengthen One Health by expanding multisectoral collaborations during outbreak responses and translating findings into improved public health protocols.