尊敬的 微信汇率:1円 ≈ 0.046166 元 支付宝汇率:1円 ≈ 0.046257元 [退出登录]
SlideShare a Scribd company logo
Batrachochytrium dendrobatidis
Batrachochytrium dendrobatidis
Bd
Janthinobacterium lividum
Pseudomonas fluorescens,
Bd in vitro. Bd
Lithobates sphenocephalus
J. lividum P.fluorescens.
, Bd-
J. lividum P. fluorescens. Bd
J. lividum
P. fluorescens Bd- . sphenocephalus
Bd
L. sphenocephalus Bd
Bd
Bd
Bd
Bd
Bd- . sphenocephalus
Bd
Bd
Bd
Bd
L. sphenocephalus L. sphenocephalus
Janthinobacterium lividum Pseudomonas fluorescens
L. sphenocephalus L. sphenocephalus
L. sphenocephalus Bd
Bd
L. sphenocephalus Bd Bd
J. lividum P. fluorescens L. sphenocephalus
L. sphenocephalus Bd L. sphenocephalus
J. lividum P. fluorescens Bd
Chapter 1: Exposure of Lithobates sphenocephalus to probiotic
bacteria for colonization
Introduction
Conceptual Framework
Batrachochytrium
Bd
,
Bd
Bd
Bd
et al.
Bd
et al.
Bd Bd,
Bd- Bd
Bd
Bd
Bd
Plethodon
cinereus, Bd
Janthinobacterium lividum Pseudomonas fluorescens,
Bd .
Bd Bd
Bd
J. lividum P. fluorescens
et al. et al. ,
Bd
Bd
Lithobates
sphenocephalus Bd
Bd
Bd
Bd
Literature Review
Bd
Bd-
Bd
J. lividum P. fluorescens L. sphenocephalus
Bd,
. J. lividum
P. cinereus Bd
Bd
Bd
Bd
Bd Bd
P. fluorescens J. lividum, Bd
Rana muscosa Rana muscosa
in vivo,
Bd J.
lividum P. fluorescen
Bd .
L. sphenocephalus
J. lividum P. fluorescens
. .
Bd
P. fluorescens J. lividum Bd
P. fluorescens
Rhizoctonia solani
Bacillus harveyi
Vibrio
L.
sphenocephalus, J. lividum P. fluorescens
.
in vivo
Bd
L. sphenocephalus J. lividum P. fluorescens
J. lividum Rana
muscosa Bd
Bd.
bd
L. sphenocephalus Bd
L. sphenocephalus
Bd L.
sphenocephalus
J. lividum P. fluorescens L.
sphenocephalus
J. lividum P. fluorescens Bd
L. sphenocephalus J. lividum P.
fluorescens
L. sphenocephalus
Hypotheses
J. lividum P. fluorescens L. sphenocephalus
J. lividum P. fluorescens L. sphenocephalus L.
sphenocephalus
L. sphenocephalus J.
lividum P. fluorescens
Methodology
Animal Husbandry
L. sphenocephalus
Bd
L. sphenocephalus
Probiotic Cultures
J. lividum P. fluorescens
, ,
.
J. lividum P. fluorescens
J. lividum P.
fluorescens L. sphenocephalus
J. lividum
P. fluorescens
L. sphenocephalus
AMP Collection, Purification, and Efficacy
L. sphenocephalus
L.
sphenocephalus
L. sphenocephalus
J. lividum
P. fluorescens
.
Bd
L. sphenocephalus J. lividum P. fluorescens
J. lividum P.
fluorescens, .
J. lividum P.
fluorescens L. sphenocephalus.
L. sphenocephalus
Probiotic Exposure and Quantification
,
1) J. lividum+/P. fluorescens+
2) J. lividum+/ P. fluorescens; 3) J.
lividum/P. fluorescens+ 4) J. lividum P. fluorescens
.
Statistical Analyses
N =
Results
L. sphenocephalus J. lividum
P. fluorescens
Comparisons of Bacterial Cell Concentrations over Time
J. lividum F = p
J. lividum
L. sphenocephalus
F p
Figure 1 L. sphenocephalus
L. sphenocephalus
J. lividum P. fluorescens
p p p
L. sphenocephalus
J. lividum P. fluorescens.
J. lividum
P. fluorescens F . p P.
fluorescens L. sphenocephalus J.
lividum
AMP production in response to bioaugmentation
F p
J. lividum P. fluorescens
Figure 2 L. sphenocephalus F
P
L. sphenocephalus
Bd
F p
L. sphenocephalus
p
p
p
in
vitro
Discussion
Findings and Conclusions
L. sphenocephalus
J. lividum P. fluorescen
Bd.
L. sphenocephalus
L. sphenocephalus
J. lividum P. fluorescens,
, ,
J. lividum P. fluorescens L. sphenocephalus
J. lividum Bd
P. fluorescens
Bd
L. sphenocephalus
L. sphenocephalus
L. sphenocephalus
J. lividum P. fluorescens
P. fluorescens L. sphenocephalus,
J. lividum. P. fluorescens Bd
J. lividum,
P. fluorescens
L. sphenocephalus
Bd
P.
fluorescens Bd
P. fluorescens J. lividum,
Bd J. lividum
Bd,
Recommendations for Future Research
J. lividum P. fluorescens
L. sphenocephalus,
Bd.
Bd
Bd
Bd-
in vivo
Bd
Bd
Chapter 2: Susceptibility to Bd after Successful Colonization of
L. sphenocephalus with J. lividum and
P. fluorescens
Introduction
Conceptual Framework
Batrachochytrium dendrobatidis Bd
Bd
Bd
Bd
Bd
Bd
Bd
Xenopus laevis) Bd
Bd .
Bd
Literature Review
Bd
Bd
.
Janthinobacterium lividum, Bd
J. lividum
Bd Bd
J. lividum
Bd, .
Pseudomonas fluorescens Bd in vitro
P.
fluorescens
J. lividum P. fluorescens
Bd
.
Bd
Bd,
Bd
. Bd
Bd
Bd
Bd.
.
Bd
Bd
.
Bd
Bd
. Bd ,
Bd
et al.
Lithobates sphenocephalus;
.
Bd-
Plethodon cinereus, J. lividum
Bd
J. lividum
P.fluorescens, Bd
Lithobates sphenocephalus J. lividum P. fluorescens
,
L. sphenocephalus
J.lividum P.fluorescens.
Bd L.
sphenocephalus
Bd-
J. lividum P. fluorescens Bd ,
L. sphenocephalus Bd.
Hypotheses
J. lividum P. fluorescens L. sphenocephalus
Bd
Bd
Bd
in vitro et al. .
L. sphenocephalus Bd
J. lividum P. fluorescens
L. sphenocephalus
J. lividum P. fluorescens Bd
Methodology
Exposure of L. sphenocephalus to Bd
L. sphenocephalus
Bd
L. sphenocephalus.
Bd L.
sphenocephalus Bd
L.
sphenocephalus.
Bd
Bd
Bd
Bd
Bd Bd
L.
sphenocephalus Bd
Bd
J. lividum P. fluorescens J. lividum P. fluorescens +
J. lividum, P. fluorescens, Bd
Bd
Bd
L. sphenocephalus
Bd
Probiotic and Bd quantification
L. sphenocephalus Bd
J. lividum
P.fluorescens
J. lividum P.
fluorescens
Bd
Bd
L. sphenocephalus Bd-
Bd
AMP Collection, Purification, and Quantification
Bd
L. sphenocephalus
.
L. sphenocephalus
Quantitative Polymerase Chain reaction (qPCR) Analysis
Bd
Bd
Bd
Bd
Bd
Bd
Statistical Analyses
L. sphenocephalus
N
N
Bd
Bd Bd
Bd
Results
Bd-induced Changes of AMP Production
L. sphenocephalus F
= p F = p
Bd F p
.
Bd P. fluorescens/Bd F = p
J.lividum/Bd F p
Bd
L. sphenocephalus J.lividum P. fluorescens .
Bd L.
sphenocephalus
F4,36 p F8, 36 = p
Bacterial Concentrations Fluctuates Post-Bd Infection
F p
L. sphenocephalus
F p
Figure 3
Figure 4. L. sphenocephalus
P
Bd
F p J. lividum F1,7 p P. fluorescens F
p
Probiotic Inhibition of Bd zoospores
Bd
F1,4 p
Bd L. sphenocephalus
J. lividum
Bd
F p
J. lividum P.
fluorescens p L.
sphenocephalus J. lividum Bd
P. fluorescens p Bd
J.lividum p
Bd
Figure 5 L. sphenocephalus
Discussion
Discussion of Findings and Conclusions
Bd
J. lividum P. fluorescens Bd
L. sphenocephalus
L. sphenocephalus
J.lividum P. fluorescens
J. lividum P.
fluorescens
Bd . J. lividum
P.fluorescens
L. sphenocephalus
.
L. sphenocephalus
J.lividum P. fluorescens
L. sphenocephalus
Bd-
J. lividum P. fluorescens,
L. sphenocephalus
Bd
L. sphenocephalus Bd
J. lividum P. fluorescens
L. sphenocephalus J. lividum P.
fluorescens, Bd
L.
sphenocephalus
L. sphenocephalus
.
J. lividum P. fluorescens
Bd- L.
sphenocephalus J. lividum P. fluorescens
K
L.
sphenocephalus
et al. .
J. lividum P. fluorescens
L.
sphenocephalus Bd
Bd, L. sphenocephalus
Bd-
Bd
Bd L. sphenocephalus
Bd
J. lividum P.
fluorescens
J. lividum P. fluorescens
Flavobacterium johnsoniae and P. fluorescens Bd- Alytes
obstetricans
Recommendations for future research
Bd
.
.
Bd
J. lividum P. fluorescens Bd L.
sphenocephalus
. in vivo
L. sphenocephalus,
Bd
Bd
Bd
Bd
.
Bd
Bd
L. sphenocephalus J. lividum P. fluorescens
L. sphenocephalus
J. lividum P. fluorescens.
Bd
Daphnia magna Bd
L. sphenocephalus
D. magna L. sphenocephalus
Bd.
Bd.
J. lividum P. fluorescens
Bd
L. sphenocephalus with J. lividum P. fluorescens
Bd .
Chapter 3: Addition of J. lividum and P. fluorescens to
BD-Infected L. sphenocephalus
Introduction
Conceptual Framework
.
.
Batrachochytrium
dendrobatidis Bd Bd
Bd Bd
Bd
Bd
Bd-
Bd
Bd-
Bd
.
Bd .
. .
Bd
Janthinobacterium lividum
Bd .
Hyla spp.
et al.
Literature review
Bd J. lividum Pseudomonas fluorescens,
in vitro
Bd Bd
J. lividum Bd
P. fluorescens,
Bd
J. lividum
P. fluorescens Bd
Bd
Bd
Bd-
J. lividum P.
fluorescens,
J. lividum P.
fluorescens Bd
Lithobates sphenocephalus in
vitro. L. sphenocephalus Bd
J. lividum P. fluorescens
Bd .
Bd , L. sphenocephalus J. lividum P. fluorescens
Bd
J. lividum P. fluorescens Bd
Bd
Bd J. lividum P. fluorescens Bd
L. sphenocephalus Bd
Hypotheses
J. lividum P. fluorescens L. sphenocephalus
Bd Bd L. sphenocephalus
J. lividum P. fluorescens. Bd
L. sphenocephalus
.
Bd
L. sphenocephalus
Methodology
Bacterial Exposure of Bd-Infected L. sphenocephalus
Acheta domestica
Bd
L. sphenocephalus.
Bd
Bd
Bd
Bd
L.
sphenocephalus Bd
Bd
J. lividum
P. fluorescens J. lividum+/P. fluorescens+ J. lividum+/P. fluorescens-
J. lividum-/P. fluorescens +. Bd
Bd J. lividum P.
fluorescens.
Bd
Bd and probiotic quantification
L. sphenocephalus
Bd
Bd
Bd
J lividum P. fluorescens
J. lividum P.
fluorescens
J.
lividum P. fluorescens
AMP collection, purification and quantification
Bd
L. sphenocephalus
.
Statistical Analyses
L. sphenocephalus
N
N
Bd
Results
Bd infection effects on bacterial colonization
J. lividum P. fluorescens L. sphenocephalus
Bd
L.
sphenocephalus J.lividum P. fluorescens.
Bd .
J. lividum P. fluorescens
J. lividum P. fluorescens N
F
p
AMP release during Bd infection and subsequent bacterial exposure
Bd F3,22 p
F3,22 p
F p
F3,22 p Bd
L. sphenocephalus
F p
Bd zoospore loads in response to probiotic intervention
Bd- Bd
L. sphenocephalus
J. lividum P. fluorescens Bd
F p
Bd
Bd J. lividum P. fluorescens
Bd-
J. lividum P. fluorescens L. sphenocephalus
Bd
F p
F p Bd
Bd
Discussion
Discussion of findings, conclusions
Bd- L.
sphenocephalus, J. lividum P. fluorescens
. L. sphenocephalus
J.lividum P.
fluorescens Bd-
.
L. sphenocephalus
L.
sphenocephalus
Bd
Bd
J. lividum P. fluorescens
L. sphenocephalus Bd
Bd L. sphenocephalus
J. lividum P.
fluorescens
.
L. sphenocephalus
J. lividum P. fluorescens.
. Bd
Bd L. sphenocephalus
Bd
Bd
L.
sphenocephalus Bd
J.lividum
P.fluorescens
Bd, Bd
Bd
L. sphenocephalus Bd
et al. et al . Lithobates clamitans
Bd ,
L. sphenocephalus
J. lividum P.
fluorescens L. sphenocephalus Bd
Bd
L. sphenocephalus Bd
Bd
J. lividum P. fluorescens Bd
Bd
Bd,
Bd
Bd,
Bd Bd
Recommendations for future research
Bd Bd
.
Bd Bd
in vitro
.
Bd
Bd
Bd
Bd
bd
Bd-
L. sphenocephalus Bd .
J. lividum P. fluorescens
.
Bd
Bd
Bd
J. lividum P.fluorescens
Staphylococcus spp.,
Bd-
L. sphenocephalus
J. lividum
P.fluorescens
J. lividum P. fluorescens Bd
J. lividum P. fluorescens
Bd L. sphenocephalus
L. sphenocephalus Bd
Bd
Bd
L. sphenocephalus Bd
.
Bd-
Bd-
Pseudomonas fluorescens Journal of
Bacteriology
Batrachochytrium
dendrobatidis Molecular Ecology 22
Applied and
Environmental Microbiology, 75(21), 6635-6638.
PLoS One 5
Ecohealth 8
Frontiers in Ecology and the Environment 5.10,
The role of cutaneous bacteria in resistance of Australian tropical
rainforest frogs to the amphibian chytrid fungus Batrachochytrium
dendrobatidis
Gesunde Pflanzen 59
Diseases of Aquatic
Organisms 68
Diseases of Aquatic Organisms 68
Ecology Letters.
Hyla cinerea
Batrachochytrium dendrobatidis PloS One 7
Batrachochytrium dendrobatidis
PloS one 8
Ecology, 86,
Plethodon
cinereus. Journal of Chemical Ecology
Janthinobacterium lividum
Plethodon cinereus Journal of Chemical Ecology 34
Scientific
reports
Batrachochytrium dendrobatidis
Fungal Genetics and
Biology, 49,
Disease ecology: community structure and
pathogen dynamics
General and
Comparative Endocrinology 176
Proceedings of the National Academy of
Sciences 107
Herpetologica, 63,
Environmental Science & Technology 41
Molecular Biology and Evolution 19
Biotechnology and
Applied Biochemistry 48
Rana muscosa Copeia 2001
Journal of Zoo and
Wildlife Medicine 39
Conservation Biology 26
Diseases of Aquatic Organisms 72
Batrachochytrium dendrobatidis
Oikos 118
Pseudomonas fluorescens
Saccharomyces cerevisiae Antonie van Leeuwenhoek 97
Batrachochytrium dendrobatidis Xenopus laevis Ecotoxicology
and Environmental Safety 71
Nature Reviews Microbiology 10
Freshwater Biology 57
Diseases of Aquatic Organisms 83
The ISME Journal 3
Food Control, 32
Archives of Environmental Health: An
International Journal 46
Proceedings of the National Academy of Sciences
Batrachochytrium dendrobatidis
Emerging Infectious
Diseases 9
Functional Ecology 22
Pseudomonas fluorescens Applied and Environmental
Microbiology 76
Batrachochytrium dendrobatidis
Diseases of Aquatic Organisms 77
Lactobacillus
rhamnosus Lactobacillus reuteri
Candida albicans. Infectious Diseases in Obstetrics and
Gynecology 2012
PloS one 9
y.
Journal of Archaeological Method and Theory 17 4
Batrachochytrium dendrobatidis
Rana muscosa Biological
Conservation, 143
Batrachochytrium
dendrobatidis PloS one 8
Copeia
Microbial Ecology 2
Lithobates
catesbeianus Mycopathologia
Microbiology: Laboratory theory and
application
PLoS biology 6
Frontiers in
Microbiology 5
The ISME journal 8
Gastroenterology, 121(3),
Applied and Environmental Microbiology 70
The ISME Journal 6
Journal of Hygiene, 38
Annual Reviews in Microbiology,
3,
Aquaculture 164
Batrachochytrium dendrobatidis Biological
Conservation 152
Batrachochytrium dendrobatidis
Rana muscosa Journal of
Chemical Ecology 38
Nature Protocols 1
Niche construction: the
neglected process in evolution
Janthinobacterium lividum Journal of
Applied Microbiology 102
Ecology 85
Rana pipiens
Global Change Biology 18
The Journal of
Experimental Biology 216
Batrachochytrium dendrobatidis
Journal of Wildlife Diseases 43
Batrachochytrium dendrobatidis
Diseases of Aquatic Organisms 75
Bioscience 59
Microbiology and
Molecular Biology Reviews 74
PLoS genetics 9
Immunological reviews 166
Batrachochytrium dendrobatidis
Developmental & Comparative
Immunology 26
Developmental & Comparative Immunology 29
Rana muscosa Developmental &
Comparative Immunology 30
Biochimica et Biophysica Acta (BBA)-Biomembranes 1788
Proceedings of the National Academy of
Sciences 105
Batrachochytrium dendrobatidis Diseases of
Aquatic Organisms 82
Journal of
Herpetology
Proceedings of the National Academy of Sciences,
108(40), .
Aquaculture 256
Ambystoma tigrinum
Journal of Wildlife Diseases 44
Fusarium oxysporum Aspergillus Pseudomonas fluorescens
Journal of Pharmacy and
Biological Science 1
The ecology of the nitrogen cycle
Rana pipiens
Developmental & Comparative
Immunology 33
Environmental Toxicology and Chemistry, 16(2),
Oecologia 93
Journal of Parasitology 96
Diseases of Aquatic
Organisms 77
Batrachochytrium dendrobatidis
Microbes and Infection 13
Batrachochytrium dendrobatidis
Fungal Ecology 4
Host-pathogen dynamics of
amphibian chytridiomycosis: the role of the skin microbiome in health and
disease.
Biotropica 43
The ISME journal
Batrachochytrium dendrobatidis Diseases of Aquatic
Organisms, 77
Rana
Diseases of aquatic organisms 98
PloS one 9
Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response
Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response
Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response
Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response
Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response
Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response
Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response

More Related Content

Viewers also liked

MY midsummer
MY midsummerMY midsummer
MY midsummer
Angel Fox
 
Juan silva
Juan silvaJuan silva
Juan silva
juan18dj
 
Power
PowerPower
Power
39641010
 
FORRES Lookbook
FORRES LookbookFORRES Lookbook
FORRES Lookbook
NABILLA PUTRI
 
Instagram.pptx
Instagram.pptxInstagram.pptx
Instagram.pptx
anto occhoa
 
Kone eco space
Kone eco spaceKone eco space
Kone eco space
Roland265
 
Trompeta bastarda
Trompeta bastardaTrompeta bastarda
Trompeta bastarda
Manuel Arnedo
 
final project- PD
final project- PDfinal project- PD
final project- PD
shirel shimonov
 
University of Memphis Newletter
University of Memphis NewletterUniversity of Memphis Newletter
University of Memphis Newletter
Alissa (crissimi)
 
Final
FinalFinal
Actividaddeaprendizaje08
Actividaddeaprendizaje08Actividaddeaprendizaje08
Actividaddeaprendizaje08
Brianvega10-12
 
6 Home Décor Ideas That Your Kids Would Absolutely Love
6 Home Décor Ideas That Your Kids Would Absolutely Love6 Home Décor Ideas That Your Kids Would Absolutely Love
6 Home Décor Ideas That Your Kids Would Absolutely Love
Pinclick
 
Chino mandarin
Chino mandarinChino mandarin
Chino mandarin
bajas987
 
Presentación1- tejedohh
Presentación1- tejedohhPresentación1- tejedohh
Presentación1- tejedohh
Victor Tejedohh
 
Slide Show Handout SASS2010
Slide Show Handout SASS2010Slide Show Handout SASS2010
Slide Show Handout SASS2010
Joe Mason
 
Presentation-LinkedIn
Presentation-LinkedInPresentation-LinkedIn
Presentation-LinkedIn
David Alavi, M.Sc., E.I.T.
 

Viewers also liked (17)

MY midsummer
MY midsummerMY midsummer
MY midsummer
 
presentatie COSV v2
presentatie COSV v2presentatie COSV v2
presentatie COSV v2
 
Juan silva
Juan silvaJuan silva
Juan silva
 
Power
PowerPower
Power
 
FORRES Lookbook
FORRES LookbookFORRES Lookbook
FORRES Lookbook
 
Instagram.pptx
Instagram.pptxInstagram.pptx
Instagram.pptx
 
Kone eco space
Kone eco spaceKone eco space
Kone eco space
 
Trompeta bastarda
Trompeta bastardaTrompeta bastarda
Trompeta bastarda
 
final project- PD
final project- PDfinal project- PD
final project- PD
 
University of Memphis Newletter
University of Memphis NewletterUniversity of Memphis Newletter
University of Memphis Newletter
 
Final
FinalFinal
Final
 
Actividaddeaprendizaje08
Actividaddeaprendizaje08Actividaddeaprendizaje08
Actividaddeaprendizaje08
 
6 Home Décor Ideas That Your Kids Would Absolutely Love
6 Home Décor Ideas That Your Kids Would Absolutely Love6 Home Décor Ideas That Your Kids Would Absolutely Love
6 Home Décor Ideas That Your Kids Would Absolutely Love
 
Chino mandarin
Chino mandarinChino mandarin
Chino mandarin
 
Presentación1- tejedohh
Presentación1- tejedohhPresentación1- tejedohh
Presentación1- tejedohh
 
Slide Show Handout SASS2010
Slide Show Handout SASS2010Slide Show Handout SASS2010
Slide Show Handout SASS2010
 
Presentation-LinkedIn
Presentation-LinkedInPresentation-LinkedIn
Presentation-LinkedIn
 

Interactions Among Bacteria and Batrachochytrium dendrobatidis- An Investigation of the Amphibian Host Immune Response

  • 1.
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7. Batrachochytrium dendrobatidis Batrachochytrium dendrobatidis Bd Janthinobacterium lividum Pseudomonas fluorescens, Bd in vitro. Bd Lithobates sphenocephalus J. lividum P.fluorescens. , Bd- J. lividum P. fluorescens. Bd J. lividum P. fluorescens Bd- . sphenocephalus Bd
  • 10. L. sphenocephalus L. sphenocephalus Janthinobacterium lividum Pseudomonas fluorescens L. sphenocephalus L. sphenocephalus L. sphenocephalus Bd Bd L. sphenocephalus Bd Bd J. lividum P. fluorescens L. sphenocephalus L. sphenocephalus Bd L. sphenocephalus J. lividum P. fluorescens Bd
  • 11. Chapter 1: Exposure of Lithobates sphenocephalus to probiotic bacteria for colonization Introduction Conceptual Framework Batrachochytrium Bd , Bd Bd Bd et al.
  • 12. Bd et al. Bd Bd, Bd- Bd Bd Bd Bd Plethodon cinereus, Bd Janthinobacterium lividum Pseudomonas fluorescens, Bd . Bd Bd Bd J. lividum P. fluorescens et al. et al. , Bd Bd Lithobates sphenocephalus Bd Bd
  • 13. Bd Bd Literature Review Bd Bd- Bd J. lividum P. fluorescens L. sphenocephalus Bd, . J. lividum P. cinereus Bd Bd Bd Bd
  • 14. Bd Bd P. fluorescens J. lividum, Bd Rana muscosa Rana muscosa in vivo, Bd J. lividum P. fluorescen Bd . L. sphenocephalus J. lividum P. fluorescens . . Bd P. fluorescens J. lividum Bd P. fluorescens Rhizoctonia solani Bacillus harveyi Vibrio L. sphenocephalus, J. lividum P. fluorescens
  • 15. . in vivo Bd L. sphenocephalus J. lividum P. fluorescens J. lividum Rana muscosa Bd Bd. bd L. sphenocephalus Bd L. sphenocephalus Bd L. sphenocephalus J. lividum P. fluorescens L. sphenocephalus J. lividum P. fluorescens Bd L. sphenocephalus J. lividum P. fluorescens
  • 16. L. sphenocephalus Hypotheses J. lividum P. fluorescens L. sphenocephalus J. lividum P. fluorescens L. sphenocephalus L. sphenocephalus L. sphenocephalus J. lividum P. fluorescens Methodology Animal Husbandry L. sphenocephalus Bd L. sphenocephalus
  • 17. Probiotic Cultures J. lividum P. fluorescens , , . J. lividum P. fluorescens J. lividum P. fluorescens L. sphenocephalus
  • 18. J. lividum P. fluorescens L. sphenocephalus AMP Collection, Purification, and Efficacy L. sphenocephalus L. sphenocephalus L. sphenocephalus J. lividum P. fluorescens .
  • 19. Bd L. sphenocephalus J. lividum P. fluorescens J. lividum P. fluorescens, . J. lividum P. fluorescens L. sphenocephalus. L. sphenocephalus Probiotic Exposure and Quantification , 1) J. lividum+/P. fluorescens+ 2) J. lividum+/ P. fluorescens; 3) J. lividum/P. fluorescens+ 4) J. lividum P. fluorescens .
  • 20. Statistical Analyses N = Results L. sphenocephalus J. lividum P. fluorescens Comparisons of Bacterial Cell Concentrations over Time J. lividum F = p J. lividum L. sphenocephalus F p
  • 21. Figure 1 L. sphenocephalus L. sphenocephalus J. lividum P. fluorescens p p p L. sphenocephalus J. lividum P. fluorescens. J. lividum P. fluorescens F . p P. fluorescens L. sphenocephalus J. lividum AMP production in response to bioaugmentation F p
  • 22. J. lividum P. fluorescens Figure 2 L. sphenocephalus F P L. sphenocephalus Bd F p L. sphenocephalus p p p
  • 23. in vitro Discussion Findings and Conclusions L. sphenocephalus J. lividum P. fluorescen Bd. L. sphenocephalus L. sphenocephalus J. lividum P. fluorescens, , , J. lividum P. fluorescens L. sphenocephalus J. lividum Bd P. fluorescens Bd
  • 24. L. sphenocephalus L. sphenocephalus L. sphenocephalus J. lividum P. fluorescens P. fluorescens L. sphenocephalus, J. lividum. P. fluorescens Bd J. lividum, P. fluorescens L. sphenocephalus Bd P. fluorescens Bd P. fluorescens J. lividum,
  • 25. Bd J. lividum Bd, Recommendations for Future Research J. lividum P. fluorescens L. sphenocephalus, Bd. Bd Bd Bd- in vivo Bd Bd
  • 26. Chapter 2: Susceptibility to Bd after Successful Colonization of L. sphenocephalus with J. lividum and P. fluorescens Introduction Conceptual Framework Batrachochytrium dendrobatidis Bd Bd Bd Bd Bd Bd Bd Xenopus laevis) Bd
  • 27. Bd . Bd Literature Review Bd Bd . Janthinobacterium lividum, Bd J. lividum Bd Bd J. lividum Bd, . Pseudomonas fluorescens Bd in vitro P. fluorescens J. lividum P. fluorescens Bd .
  • 30. J. lividum P.fluorescens, Bd Lithobates sphenocephalus J. lividum P. fluorescens , L. sphenocephalus J.lividum P.fluorescens. Bd L. sphenocephalus Bd- J. lividum P. fluorescens Bd , L. sphenocephalus Bd. Hypotheses J. lividum P. fluorescens L. sphenocephalus Bd Bd Bd in vitro et al. . L. sphenocephalus Bd J. lividum P. fluorescens L. sphenocephalus J. lividum P. fluorescens Bd
  • 31. Methodology Exposure of L. sphenocephalus to Bd L. sphenocephalus Bd L. sphenocephalus. Bd L. sphenocephalus Bd L. sphenocephalus. Bd
  • 32. Bd Bd Bd Bd Bd L. sphenocephalus Bd Bd J. lividum P. fluorescens J. lividum P. fluorescens + J. lividum, P. fluorescens, Bd Bd Bd L. sphenocephalus Bd Probiotic and Bd quantification L. sphenocephalus Bd J. lividum P.fluorescens
  • 33. J. lividum P. fluorescens Bd Bd L. sphenocephalus Bd- Bd AMP Collection, Purification, and Quantification Bd L. sphenocephalus .
  • 34. L. sphenocephalus Quantitative Polymerase Chain reaction (qPCR) Analysis Bd Bd Bd Bd Bd Bd Statistical Analyses L. sphenocephalus N N Bd
  • 35. Bd Bd Bd Results Bd-induced Changes of AMP Production L. sphenocephalus F = p F = p Bd F p . Bd P. fluorescens/Bd F = p J.lividum/Bd F p Bd L. sphenocephalus J.lividum P. fluorescens . Bd L. sphenocephalus F4,36 p F8, 36 = p Bacterial Concentrations Fluctuates Post-Bd Infection F p L. sphenocephalus
  • 36. F p Figure 3 Figure 4. L. sphenocephalus P Bd
  • 37. F p J. lividum F1,7 p P. fluorescens F p Probiotic Inhibition of Bd zoospores Bd F1,4 p Bd L. sphenocephalus J. lividum Bd F p J. lividum P. fluorescens p L. sphenocephalus J. lividum Bd P. fluorescens p Bd J.lividum p Bd
  • 38. Figure 5 L. sphenocephalus Discussion Discussion of Findings and Conclusions Bd J. lividum P. fluorescens Bd L. sphenocephalus L. sphenocephalus J.lividum P. fluorescens J. lividum P. fluorescens Bd . J. lividum P.fluorescens L. sphenocephalus . L. sphenocephalus
  • 39. J.lividum P. fluorescens L. sphenocephalus Bd- J. lividum P. fluorescens, L. sphenocephalus Bd L. sphenocephalus Bd J. lividum P. fluorescens L. sphenocephalus J. lividum P. fluorescens, Bd L. sphenocephalus L. sphenocephalus .
  • 40. J. lividum P. fluorescens Bd- L. sphenocephalus J. lividum P. fluorescens K L. sphenocephalus et al. . J. lividum P. fluorescens L. sphenocephalus Bd Bd, L. sphenocephalus Bd- Bd Bd L. sphenocephalus Bd J. lividum P. fluorescens J. lividum P. fluorescens Flavobacterium johnsoniae and P. fluorescens Bd- Alytes obstetricans
  • 41. Recommendations for future research Bd . . Bd J. lividum P. fluorescens Bd L. sphenocephalus . in vivo L. sphenocephalus, Bd Bd Bd Bd . Bd
  • 42. Bd L. sphenocephalus J. lividum P. fluorescens L. sphenocephalus J. lividum P. fluorescens. Bd Daphnia magna Bd L. sphenocephalus D. magna L. sphenocephalus Bd. Bd. J. lividum P. fluorescens Bd L. sphenocephalus with J. lividum P. fluorescens Bd .
  • 43. Chapter 3: Addition of J. lividum and P. fluorescens to BD-Infected L. sphenocephalus Introduction Conceptual Framework . . Batrachochytrium dendrobatidis Bd Bd Bd Bd Bd
  • 45. et al. Literature review Bd J. lividum Pseudomonas fluorescens, in vitro Bd Bd J. lividum Bd P. fluorescens, Bd J. lividum P. fluorescens Bd Bd Bd Bd-
  • 46. J. lividum P. fluorescens, J. lividum P. fluorescens Bd Lithobates sphenocephalus in vitro. L. sphenocephalus Bd J. lividum P. fluorescens Bd . Bd , L. sphenocephalus J. lividum P. fluorescens Bd J. lividum P. fluorescens Bd Bd Bd J. lividum P. fluorescens Bd
  • 47. L. sphenocephalus Bd Hypotheses J. lividum P. fluorescens L. sphenocephalus Bd Bd L. sphenocephalus J. lividum P. fluorescens. Bd L. sphenocephalus . Bd L. sphenocephalus Methodology Bacterial Exposure of Bd-Infected L. sphenocephalus Acheta domestica Bd L. sphenocephalus. Bd Bd
  • 48. Bd Bd L. sphenocephalus Bd Bd J. lividum P. fluorescens J. lividum+/P. fluorescens+ J. lividum+/P. fluorescens- J. lividum-/P. fluorescens +. Bd Bd J. lividum P. fluorescens. Bd Bd and probiotic quantification L. sphenocephalus
  • 49. Bd Bd Bd J lividum P. fluorescens J. lividum P. fluorescens J. lividum P. fluorescens AMP collection, purification and quantification Bd
  • 50. L. sphenocephalus . Statistical Analyses L. sphenocephalus N N Bd Results Bd infection effects on bacterial colonization J. lividum P. fluorescens L. sphenocephalus
  • 51. Bd L. sphenocephalus J.lividum P. fluorescens. Bd . J. lividum P. fluorescens J. lividum P. fluorescens N F p AMP release during Bd infection and subsequent bacterial exposure Bd F3,22 p F3,22 p F p F3,22 p Bd L. sphenocephalus F p
  • 52. Bd zoospore loads in response to probiotic intervention Bd- Bd L. sphenocephalus J. lividum P. fluorescens Bd F p Bd Bd J. lividum P. fluorescens Bd- J. lividum P. fluorescens L. sphenocephalus Bd F p F p Bd Bd Discussion Discussion of findings, conclusions Bd- L. sphenocephalus, J. lividum P. fluorescens . L. sphenocephalus J.lividum P. fluorescens Bd- . L. sphenocephalus
  • 53. L. sphenocephalus Bd Bd J. lividum P. fluorescens L. sphenocephalus Bd Bd L. sphenocephalus J. lividum P. fluorescens . L. sphenocephalus J. lividum P. fluorescens. . Bd Bd L. sphenocephalus Bd Bd L. sphenocephalus Bd
  • 54. J.lividum P.fluorescens Bd, Bd Bd L. sphenocephalus Bd et al. et al . Lithobates clamitans Bd , L. sphenocephalus J. lividum P. fluorescens L. sphenocephalus Bd Bd L. sphenocephalus Bd Bd J. lividum P. fluorescens Bd Bd Bd, Bd Bd, Bd Bd
  • 55. Recommendations for future research Bd Bd . Bd Bd in vitro . Bd Bd Bd Bd bd Bd- L. sphenocephalus Bd . J. lividum P. fluorescens . Bd Bd Bd
  • 56. J. lividum P.fluorescens Staphylococcus spp., Bd- L. sphenocephalus J. lividum P.fluorescens J. lividum P. fluorescens Bd J. lividum P. fluorescens Bd L. sphenocephalus L. sphenocephalus Bd Bd Bd L. sphenocephalus Bd . Bd- Bd-
  • 57. Pseudomonas fluorescens Journal of Bacteriology Batrachochytrium dendrobatidis Molecular Ecology 22 Applied and Environmental Microbiology, 75(21), 6635-6638. PLoS One 5 Ecohealth 8 Frontiers in Ecology and the Environment 5.10, The role of cutaneous bacteria in resistance of Australian tropical rainforest frogs to the amphibian chytrid fungus Batrachochytrium dendrobatidis Gesunde Pflanzen 59 Diseases of Aquatic Organisms 68 Diseases of Aquatic Organisms 68
  • 58. Ecology Letters. Hyla cinerea Batrachochytrium dendrobatidis PloS One 7 Batrachochytrium dendrobatidis PloS one 8 Ecology, 86, Plethodon cinereus. Journal of Chemical Ecology Janthinobacterium lividum Plethodon cinereus Journal of Chemical Ecology 34 Scientific reports Batrachochytrium dendrobatidis Fungal Genetics and Biology, 49, Disease ecology: community structure and pathogen dynamics
  • 59. General and Comparative Endocrinology 176 Proceedings of the National Academy of Sciences 107 Herpetologica, 63, Environmental Science & Technology 41 Molecular Biology and Evolution 19 Biotechnology and Applied Biochemistry 48 Rana muscosa Copeia 2001 Journal of Zoo and Wildlife Medicine 39 Conservation Biology 26 Diseases of Aquatic Organisms 72 Batrachochytrium dendrobatidis Oikos 118 Pseudomonas fluorescens
  • 60. Saccharomyces cerevisiae Antonie van Leeuwenhoek 97 Batrachochytrium dendrobatidis Xenopus laevis Ecotoxicology and Environmental Safety 71 Nature Reviews Microbiology 10 Freshwater Biology 57 Diseases of Aquatic Organisms 83 The ISME Journal 3 Food Control, 32 Archives of Environmental Health: An International Journal 46 Proceedings of the National Academy of Sciences Batrachochytrium dendrobatidis Emerging Infectious Diseases 9 Functional Ecology 22
  • 61. Pseudomonas fluorescens Applied and Environmental Microbiology 76 Batrachochytrium dendrobatidis Diseases of Aquatic Organisms 77 Lactobacillus rhamnosus Lactobacillus reuteri Candida albicans. Infectious Diseases in Obstetrics and Gynecology 2012 PloS one 9 y. Journal of Archaeological Method and Theory 17 4 Batrachochytrium dendrobatidis Rana muscosa Biological Conservation, 143 Batrachochytrium dendrobatidis PloS one 8 Copeia Microbial Ecology 2 Lithobates catesbeianus Mycopathologia Microbiology: Laboratory theory and application PLoS biology 6
  • 62. Frontiers in Microbiology 5 The ISME journal 8 Gastroenterology, 121(3), Applied and Environmental Microbiology 70 The ISME Journal 6 Journal of Hygiene, 38 Annual Reviews in Microbiology, 3, Aquaculture 164 Batrachochytrium dendrobatidis Biological Conservation 152 Batrachochytrium dendrobatidis Rana muscosa Journal of Chemical Ecology 38 Nature Protocols 1 Niche construction: the neglected process in evolution
  • 63. Janthinobacterium lividum Journal of Applied Microbiology 102 Ecology 85 Rana pipiens Global Change Biology 18 The Journal of Experimental Biology 216 Batrachochytrium dendrobatidis Journal of Wildlife Diseases 43 Batrachochytrium dendrobatidis Diseases of Aquatic Organisms 75 Bioscience 59 Microbiology and Molecular Biology Reviews 74 PLoS genetics 9 Immunological reviews 166 Batrachochytrium dendrobatidis Developmental & Comparative Immunology 26
  • 64. Developmental & Comparative Immunology 29 Rana muscosa Developmental & Comparative Immunology 30 Biochimica et Biophysica Acta (BBA)-Biomembranes 1788 Proceedings of the National Academy of Sciences 105 Batrachochytrium dendrobatidis Diseases of Aquatic Organisms 82 Journal of Herpetology Proceedings of the National Academy of Sciences, 108(40), . Aquaculture 256 Ambystoma tigrinum Journal of Wildlife Diseases 44 Fusarium oxysporum Aspergillus Pseudomonas fluorescens Journal of Pharmacy and Biological Science 1 The ecology of the nitrogen cycle
  • 65. Rana pipiens Developmental & Comparative Immunology 33 Environmental Toxicology and Chemistry, 16(2), Oecologia 93 Journal of Parasitology 96 Diseases of Aquatic Organisms 77 Batrachochytrium dendrobatidis Microbes and Infection 13 Batrachochytrium dendrobatidis Fungal Ecology 4 Host-pathogen dynamics of amphibian chytridiomycosis: the role of the skin microbiome in health and disease. Biotropica 43 The ISME journal Batrachochytrium dendrobatidis Diseases of Aquatic Organisms, 77
  • 66. Rana Diseases of aquatic organisms 98 PloS one 9
  翻译: