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Alex Michael Ward
alex.m.ward88@gmail.com
415-942-3393
812 Corriente Point Drive
Redwood City, California 94065
Professional Profile
A motivated and technically skilled virologist and cell biologist seeking a research position in the
Biotech industry. I have 14 years experience developing genetic, proteomic and cell-based
approaches to study virus-host interaction and identify therapeutic targets. I am a highly
collaborative and creative scientist with solid management, mentoring and communication skills
and a strong track record of publications and independent funding.
Work History
Assistant Professor: Program in Emerging Infectious Diseases, Duke-National University of
Singapore Graduate Medical School, Jan 2014-present
Post-doctoral Research: Laboratory of Molecular Virology, Duke-National University of
Singapore Graduate Medical School, Aug 2008-Dec 2013, Research advisor: Dr. Mariano A.
Garcia-Blanco
Education
Microbiology, Ph.D.: University of Virginia, Charlottesville, Virginia, Aug 2008
Research advisors: Dr. David Rekosh and Dr. Marie-Louise Hammarskjöld
• Mechanism of action of an antisense RNA-based anti-HIV therapeutic
• Effects of cis-acting RNA elements on nonsense-mediated decay
Biology, B.A.: Reed College, Portland, Oregon, May 1998
Professional Accomplishments
• Developed novel cell-free and cell-based assays, such as:
o Generated molecular clones of HIV-1 and Enterovirus 71 isolates for reverse
genetic studies and expression of antisense RNA
o Optimized cell culture techniques for plasmid and siRNA transfection
o Developed ELISA and plaque assays for measuring viral growth
o Studied intracellular viral replication using luciferase replicon reporter systems
o Utilized qPCR and northern blot techniques for quantitative RNA assays
o Utilized ELISA and western blot techniques for quantitative protein assays
o Developed RNA chromatography platform for purification of Retrovirus,
Flavivirus, Hepacivirus and Enterovirus RNA binding proteins (lab and clinical
isolates)
o Cloned, expressed and purified proteins in bacteria and mammalian cells
• Independently managed a research team for more than four years, including regulatory
oversight, budget management, scientific mentorship, and obtaining external funding.
• Presented at local and international meetings, served as primary and contributing author
on publications, and collaborated on basic and clinical virology research projects.
Alex Michael Ward Page 2 of 4
Publications
Journal Articles
Lopez, CM, Saminathan, K, Gunaratne, J, Ward, AM, QKI binds the 5’ UTR of EV71 and is
required for EV71 replication. Manuscript in preparation.
Ward AM, Gunaratne, J, Garcia-Blanco, MA, The role of cellular exonuclease ERI3 in
Dengue virus replication. Manuscript in preparation.
Manokaran G, Finol E, Wang C, Gunaratne J, Bahl J, Ong EZ, Tan HC, Sessions OM, Ward
AM, Gubler DJ, Harris E, Garcia-Blanco MA, Ooi EE, Subgenomic RNA of dengue virus
binds tripartite motif 25 protein to inhibit interferon expression for epidemiological
fitness. Science published online 2 July 2015
Ward AM, Bidet K, Yinglin A, Ler SG, Hogue K, Blackstock W, Gunaratne J, Garcia-Blanco MA,
Quantitative mass spectrometry of DENV-2 RNA-interacting proteins reveals that the
DEAD-box RNA helicase DDX6 binds the DB1 and DB2 3' UTR structures. RNA Biology
2011 8: 1173-86
Ward AM, Rekosh D and Hammarskjöld ML, Trafficking Through the Rev/RRE Pathway is
Essential for Efficient Inhibition of HIV-1 by an Antisense RNA derived from the Envelope
Gene. Journal of Virology 2009 83: 940-52
Grossmann KF, Ward AM, Matkovic ME, Folias AE, Moses RE, S. cerevisiae has three
pathways for DNA interstrand crosslink repair. Mutation Research 2001 487: 73-83
Grossmann KF, Ward AM, Moses RE, Saccharomyces cerevisiae lacking Snm1, Rev3 or
Rad51 have a normal S-phase but arrest permanently in G2 after cisplatin treatment.
Mutation Research 2000 461: 1-13
Book Chapters
Ward, AM, Gunaratne, J, Garcia-Blanco, MA, Identification of Dengue RNA Binding Proteins
using RNA Chromatography and Quantitative Mass Spectrometry. In: Methods in
Molecular Biology 2014 1138: 253-70
Barrows, N, Bradrick, S, Bidet, K, Anwar, A, Ward, AM, Jamison, SF, Levitt, B, LeSommer, C
and Garcia-Blanco, MA, The Interface between Dengue Virus and the Human Host. In:
Dengue and Dengue Hemorrhagic Fever, 2nd
ed. Gubler, DJ et al., eds., 2014
Funding
NMRC Cooperative Basic Research Grant (CBRG)-New Investigator Grant (BNIG11nov010),
2012-2014, Defining the function of ERI3 in DENV replication and selection of RNA
aptamers that inhibit ERI3 activity. Role: PI, SGD 189,000.00
NMRC Clinician-Scientist Individual Research Grant (NMRC/CIRG/1374/2013), 2014-2017,
Defining the role of 3’ untranslated region of the dengue virus genome in determining the
viral fitness and epidemic dengue. Role: Collaborator, SGD 1,167,272.00
Alex Michael Ward Page 3 of 4
NMRC CBRG-New Investigator Grant (BNIG13nov029), 2014-2016, The molecular basis for
EV71 pathogenesis and epidemic potential. Role: Collaborator, SGD 200,000.00
NMRC CBRG (CBRG14may057), 2015-2018, Characterization of innate immune disruption
by flaviviral subgenomic RNA (sfRNA), Role: PI, SGD 1,100,000.00
NMRC CBRG-NIG (BNIG14nov007), The impact of sequence evolution in the dengue non-
coding RNA on mosquito transmission. Role: Co-Investigator, SGD 198,000.00, Pending
budget revision
Select Presentations
Oral Presentations
Ward AM, Jamison, SF, Levitt, B, Gunaratne J, Garcia-Blanco, MA, Identification and
characterization of 3’-5’ exonucleases required for dengue virus replication, 2012
RiboClub Annual Meeting, Sep 24-26, 2012, Orford, Quebec
Ward AM, Gunaratne, J, Hogue, K, Blackstock WA, Garcia-Blanco, MA, Identification of a
host cell factor that binds to the Dengue 2 untranslated region, 4th
Asia Dengue Research
Network Meeting, Dec 8-11, 2009, Singapore
Ward AM, Hammarskjöld ML and Rekosh D, Regions of the HIV-1 Rev Response Element
outside of the primary Rev binding site are required for viral replication, Workshop on
Post-Trancriptional Control by Oncogenic Viruses, Jun 24-27, 2004, Park City, Utah
Poster Presentations
Ward, AM, Jamison, S, Levitt, B, Dominski, Z, Marzluff, WF, Gunaratne, J, and Garcia-Blanco
MA, Identification and characterization of a 3’-5’ exonuclease that binds to dengue virus
RNA and is required for viral replication, Keystone Symposium on Positive Strand RNA
Viruses, Apr 28-May 3, 2013, Boston, Massachusetts
Ward, AM, Bidet, K, Yinglin, A, Gunaratne, J, Hogue, K, Blackstock, W, Garcia-Blanco, MA,
Characterization of Host Cell Proteins that Interact with the DENV-2 RNA 5’ and 3’ UTRs
reveals a role for DDX6 in packaging of viral RNA, 16th Annual Meeting of the RNA Society,
Jun 14-18, 2011, Kyoto, Japan
Ward AM, Rekosh D, and Hammarskjöld ML, The HIV-1 Rev Response Element (RRE)
significantly improves antisense RNA targeting, 14th Conference on Retroviruses and
Opportunistic Infections, Feb 25-28, 2007, Los Angeles, California
Teaching Experience
Lecturer, Cellular Mechanisms, Graduate course at the National University of Singapore and
Temasek Lifesciences Laboratory, Sep 2014-present, Lectures on: Cellular stress response
Lecturer, Principles of Infectious Diseases, Graduate course at the Duke-NUS Graduate
Medical School, Jan 2014-present, Lectures on: Virus replication, Cell biology of viruses and
Picornaviridae
Alex Michael Ward Page 4 of 4
Lecturer, Transforming Medicine: From Bench to Bedside and Beyond, Undergraduate course
at the National University of Singapore, Singapore, Sep 2011-present, Lectures on: Emerging
Infectious Diseases
Instructor (lecture and practical), Molecular Biology of the Cell, Undergraduate course at the
School of Life and Physical Sciences, PSB Academy, Singapore, May 2010-Aug 2011, Lectures
on: Cytokinesis, Cell organelles, Cell membranes, Ion channels, Protein trafficking and
secretion, Biochemical messages, Cell signaling, Cell structure, DNA structure, replication,
mutation and repair
Professional Experience
2013-present, Organizer, Progress on Research in Emerging Infectious Diseases (PREID)
meeting
2012-present, Trainee, Academic Medicine Education Institute, Duke-NUS and SingHealth
2010-present, Member, Review Editorial Board, Frontiers in Genomic Assay Technology
Scientific Awards
2007, 2005 and 2004, Young Investigator Award, Conference on Retroviruses and
Opportunistic Infections
2000-2001, National Institutes of Health Cancer Training Grant
1998, Howard Hughes Medical Institute Equipment Grant
Professional Memberships
RNA Society
American Society of Microbiology
References
Mariano A Garcia-Blanco, M.D., Ph.D.
Professor and Chair
Department of Biochemistry & Molecular
Biology
University of Texas Medical Branch
108 Basic Science Building
301 University Boulevard
Galveston, TX 77555 USA
+1 409 772-2271
maragarc@utmb.edu
Eng Eong Ooi, B.M., Ph.D.
Associate Professor and Deputy Program
Director
Program in Emerging Infectious Diseases
Duke-NUS Graduate Medical School
8 College Road
Singapore 169857
+65 6485 7238
engeong.ooi@duke-nus.edu.sg
David Rekosh, Ph.D. and Marie-Louise
Hammarskjöld, M.D., Ph.D.
Professors of Microbiology
Myles H. Thaler Center for AIDS and
Human Retrovirus Research
University of Virginia
PO Box 800734
Charlottesville, Virginia 22908 USA
+1 434 982-1599
dr4u@virginia.edu
mh7g@virginia.edu
Jayantha Gunaratne, Ph.D.
Group Leader
Quantitative Proteomics Group
Institute of Molecular and Cell Biology
61 Biopolis Drive
Proteos
Singapore 138673
+65 6586 9689
jayanthag@imcb.a-star.edu

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CV AMW 20150709

  • 1. Alex Michael Ward alex.m.ward88@gmail.com 415-942-3393 812 Corriente Point Drive Redwood City, California 94065 Professional Profile A motivated and technically skilled virologist and cell biologist seeking a research position in the Biotech industry. I have 14 years experience developing genetic, proteomic and cell-based approaches to study virus-host interaction and identify therapeutic targets. I am a highly collaborative and creative scientist with solid management, mentoring and communication skills and a strong track record of publications and independent funding. Work History Assistant Professor: Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School, Jan 2014-present Post-doctoral Research: Laboratory of Molecular Virology, Duke-National University of Singapore Graduate Medical School, Aug 2008-Dec 2013, Research advisor: Dr. Mariano A. Garcia-Blanco Education Microbiology, Ph.D.: University of Virginia, Charlottesville, Virginia, Aug 2008 Research advisors: Dr. David Rekosh and Dr. Marie-Louise Hammarskjöld • Mechanism of action of an antisense RNA-based anti-HIV therapeutic • Effects of cis-acting RNA elements on nonsense-mediated decay Biology, B.A.: Reed College, Portland, Oregon, May 1998 Professional Accomplishments • Developed novel cell-free and cell-based assays, such as: o Generated molecular clones of HIV-1 and Enterovirus 71 isolates for reverse genetic studies and expression of antisense RNA o Optimized cell culture techniques for plasmid and siRNA transfection o Developed ELISA and plaque assays for measuring viral growth o Studied intracellular viral replication using luciferase replicon reporter systems o Utilized qPCR and northern blot techniques for quantitative RNA assays o Utilized ELISA and western blot techniques for quantitative protein assays o Developed RNA chromatography platform for purification of Retrovirus, Flavivirus, Hepacivirus and Enterovirus RNA binding proteins (lab and clinical isolates) o Cloned, expressed and purified proteins in bacteria and mammalian cells • Independently managed a research team for more than four years, including regulatory oversight, budget management, scientific mentorship, and obtaining external funding. • Presented at local and international meetings, served as primary and contributing author on publications, and collaborated on basic and clinical virology research projects.
  • 2. Alex Michael Ward Page 2 of 4 Publications Journal Articles Lopez, CM, Saminathan, K, Gunaratne, J, Ward, AM, QKI binds the 5’ UTR of EV71 and is required for EV71 replication. Manuscript in preparation. Ward AM, Gunaratne, J, Garcia-Blanco, MA, The role of cellular exonuclease ERI3 in Dengue virus replication. Manuscript in preparation. Manokaran G, Finol E, Wang C, Gunaratne J, Bahl J, Ong EZ, Tan HC, Sessions OM, Ward AM, Gubler DJ, Harris E, Garcia-Blanco MA, Ooi EE, Subgenomic RNA of dengue virus binds tripartite motif 25 protein to inhibit interferon expression for epidemiological fitness. Science published online 2 July 2015 Ward AM, Bidet K, Yinglin A, Ler SG, Hogue K, Blackstock W, Gunaratne J, Garcia-Blanco MA, Quantitative mass spectrometry of DENV-2 RNA-interacting proteins reveals that the DEAD-box RNA helicase DDX6 binds the DB1 and DB2 3' UTR structures. RNA Biology 2011 8: 1173-86 Ward AM, Rekosh D and Hammarskjöld ML, Trafficking Through the Rev/RRE Pathway is Essential for Efficient Inhibition of HIV-1 by an Antisense RNA derived from the Envelope Gene. Journal of Virology 2009 83: 940-52 Grossmann KF, Ward AM, Matkovic ME, Folias AE, Moses RE, S. cerevisiae has three pathways for DNA interstrand crosslink repair. Mutation Research 2001 487: 73-83 Grossmann KF, Ward AM, Moses RE, Saccharomyces cerevisiae lacking Snm1, Rev3 or Rad51 have a normal S-phase but arrest permanently in G2 after cisplatin treatment. Mutation Research 2000 461: 1-13 Book Chapters Ward, AM, Gunaratne, J, Garcia-Blanco, MA, Identification of Dengue RNA Binding Proteins using RNA Chromatography and Quantitative Mass Spectrometry. In: Methods in Molecular Biology 2014 1138: 253-70 Barrows, N, Bradrick, S, Bidet, K, Anwar, A, Ward, AM, Jamison, SF, Levitt, B, LeSommer, C and Garcia-Blanco, MA, The Interface between Dengue Virus and the Human Host. In: Dengue and Dengue Hemorrhagic Fever, 2nd ed. Gubler, DJ et al., eds., 2014 Funding NMRC Cooperative Basic Research Grant (CBRG)-New Investigator Grant (BNIG11nov010), 2012-2014, Defining the function of ERI3 in DENV replication and selection of RNA aptamers that inhibit ERI3 activity. Role: PI, SGD 189,000.00 NMRC Clinician-Scientist Individual Research Grant (NMRC/CIRG/1374/2013), 2014-2017, Defining the role of 3’ untranslated region of the dengue virus genome in determining the viral fitness and epidemic dengue. Role: Collaborator, SGD 1,167,272.00
  • 3. Alex Michael Ward Page 3 of 4 NMRC CBRG-New Investigator Grant (BNIG13nov029), 2014-2016, The molecular basis for EV71 pathogenesis and epidemic potential. Role: Collaborator, SGD 200,000.00 NMRC CBRG (CBRG14may057), 2015-2018, Characterization of innate immune disruption by flaviviral subgenomic RNA (sfRNA), Role: PI, SGD 1,100,000.00 NMRC CBRG-NIG (BNIG14nov007), The impact of sequence evolution in the dengue non- coding RNA on mosquito transmission. Role: Co-Investigator, SGD 198,000.00, Pending budget revision Select Presentations Oral Presentations Ward AM, Jamison, SF, Levitt, B, Gunaratne J, Garcia-Blanco, MA, Identification and characterization of 3’-5’ exonucleases required for dengue virus replication, 2012 RiboClub Annual Meeting, Sep 24-26, 2012, Orford, Quebec Ward AM, Gunaratne, J, Hogue, K, Blackstock WA, Garcia-Blanco, MA, Identification of a host cell factor that binds to the Dengue 2 untranslated region, 4th Asia Dengue Research Network Meeting, Dec 8-11, 2009, Singapore Ward AM, Hammarskjöld ML and Rekosh D, Regions of the HIV-1 Rev Response Element outside of the primary Rev binding site are required for viral replication, Workshop on Post-Trancriptional Control by Oncogenic Viruses, Jun 24-27, 2004, Park City, Utah Poster Presentations Ward, AM, Jamison, S, Levitt, B, Dominski, Z, Marzluff, WF, Gunaratne, J, and Garcia-Blanco MA, Identification and characterization of a 3’-5’ exonuclease that binds to dengue virus RNA and is required for viral replication, Keystone Symposium on Positive Strand RNA Viruses, Apr 28-May 3, 2013, Boston, Massachusetts Ward, AM, Bidet, K, Yinglin, A, Gunaratne, J, Hogue, K, Blackstock, W, Garcia-Blanco, MA, Characterization of Host Cell Proteins that Interact with the DENV-2 RNA 5’ and 3’ UTRs reveals a role for DDX6 in packaging of viral RNA, 16th Annual Meeting of the RNA Society, Jun 14-18, 2011, Kyoto, Japan Ward AM, Rekosh D, and Hammarskjöld ML, The HIV-1 Rev Response Element (RRE) significantly improves antisense RNA targeting, 14th Conference on Retroviruses and Opportunistic Infections, Feb 25-28, 2007, Los Angeles, California Teaching Experience Lecturer, Cellular Mechanisms, Graduate course at the National University of Singapore and Temasek Lifesciences Laboratory, Sep 2014-present, Lectures on: Cellular stress response Lecturer, Principles of Infectious Diseases, Graduate course at the Duke-NUS Graduate Medical School, Jan 2014-present, Lectures on: Virus replication, Cell biology of viruses and Picornaviridae
  • 4. Alex Michael Ward Page 4 of 4 Lecturer, Transforming Medicine: From Bench to Bedside and Beyond, Undergraduate course at the National University of Singapore, Singapore, Sep 2011-present, Lectures on: Emerging Infectious Diseases Instructor (lecture and practical), Molecular Biology of the Cell, Undergraduate course at the School of Life and Physical Sciences, PSB Academy, Singapore, May 2010-Aug 2011, Lectures on: Cytokinesis, Cell organelles, Cell membranes, Ion channels, Protein trafficking and secretion, Biochemical messages, Cell signaling, Cell structure, DNA structure, replication, mutation and repair Professional Experience 2013-present, Organizer, Progress on Research in Emerging Infectious Diseases (PREID) meeting 2012-present, Trainee, Academic Medicine Education Institute, Duke-NUS and SingHealth 2010-present, Member, Review Editorial Board, Frontiers in Genomic Assay Technology Scientific Awards 2007, 2005 and 2004, Young Investigator Award, Conference on Retroviruses and Opportunistic Infections 2000-2001, National Institutes of Health Cancer Training Grant 1998, Howard Hughes Medical Institute Equipment Grant Professional Memberships RNA Society American Society of Microbiology References Mariano A Garcia-Blanco, M.D., Ph.D. Professor and Chair Department of Biochemistry & Molecular Biology University of Texas Medical Branch 108 Basic Science Building 301 University Boulevard Galveston, TX 77555 USA +1 409 772-2271 maragarc@utmb.edu Eng Eong Ooi, B.M., Ph.D. Associate Professor and Deputy Program Director Program in Emerging Infectious Diseases Duke-NUS Graduate Medical School 8 College Road Singapore 169857 +65 6485 7238 engeong.ooi@duke-nus.edu.sg David Rekosh, Ph.D. and Marie-Louise Hammarskjöld, M.D., Ph.D. Professors of Microbiology Myles H. Thaler Center for AIDS and Human Retrovirus Research University of Virginia PO Box 800734 Charlottesville, Virginia 22908 USA +1 434 982-1599 dr4u@virginia.edu mh7g@virginia.edu Jayantha Gunaratne, Ph.D. Group Leader Quantitative Proteomics Group Institute of Molecular and Cell Biology 61 Biopolis Drive Proteos Singapore 138673 +65 6586 9689 jayanthag@imcb.a-star.edu
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