16. identification and characterization of cancer stem cells in liver cancer guan xin-yuan

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This document summarizes research on cancer stem cells (CSCs) in hepatocellular carcinoma (HCC). It first provides background on HCC as the 5th most common cancer and 3rd leading cause of cancer death, with risk factors including HBV/HCV infection, aflatoxin B1, alcohol, and obesity. It then defines CSCs as cells within tumors that self-renew and cause heterogeneous lineages. Studies identified a CD133+ subpopulation in HCC with CSC properties like tumorigenicity, self-renewal, differentiation, and chemoresistance. Further research showed this CD133+ CSC population contributes to chemoresistance through specific pathways and can be better characterized by A

Identification and Characterization of Cancer
Stem Cells in Hepatocellular Carcinoma

Xin-yuan Guan
Department of Clinical Oncology
The University of Hong Kong

Hepatocellular Carcinoma (HCC)
HCC
5th most common cancer
3rd leading cause of cancer death
 High incidence geographic regions: Southeast
Asia, China, sub-Saharan Africa

Etiology of HCC
 HBV or HCV infection
 Aflatoxin B1 intake
 Alcohol abuse
Obesity, diabetes and nonalcoholic fatty liver
disease

Tumor-initiating cells (TICs) / Cancer stem cells (CSCs)
- CSC has been defined as a cell within a tumor that possesses the capacity to self-renew
and to cause the heterogeneous lineages of cancer cells.
- Properties of CSCs – persistent self-renewal, sustained proliferation, tumor initiation.
- Other common (but not defining) characteristics of CSCs – rarity within a tumor, stem cell
marker expression, ability to differentiate along multiple lineages, chemoresistance,
metastasis, etc.

- Origin of CSCs – largely unknown.
e.g. for leukemia and colon cancer – normal stem cells
e.g. for liver cancer – ?

Characteristics of CD133+ subpopulation in HCC
 CD133+ cells exhibit increased tumorigenic potential
 CD133+ cells exhibit an increased potential for self-renewal
 CD133+ cells exhibit a marked ability to differentiate
 CD133+ cells preferentially express “stemness” genes
 CD133+ cells are more resistant to anticancer agents, doxorubicin
and 5-fluorouracil

Summary
Characteristics of CD133+ subpopulations, isolated from HCC clinical specimens
and cell lines are consistent with the predicted behavior of cancer stem cells.
Ma et al., Gastroenterology 2007

CD133+ CSCs contribute to HCC chemoresistance through activation of the
specific Akt/PKB and Bcl-2 survival pathway.

Ma et al., Oncogene 2008

Aldehyde dehydrogenase (ALDH) is preferentially expressed in CD133+ cells and
can be used to better characterize the CD133+ HCC CSC population.
Ma et al., Mole Cancer Res 2008

miR-130b is overexpressed in CD133+ CSCs and regulates tumor growth and selfrenewal via tumor protein TP53-INP1.
Ma et al., Cell Stem Cell 2010
CD133+ liver CSCs promote tumor angiogenesis through activation of the NTS
/ IL-8 / CXCL1 signaling cascade via MAPK signaling.

Tang KH, et al. Hepatology 2011

Knowckdown of CD133 can inhibit tumorigenicity of HCC cell lines.
Tang KH, et al. Hepatology 2011

Acknowledgments
Department of Clinical Oncology, HKU
Dr. Stephanie Ma
Ms. Yangyang Song
Ms. Carol Tong

Dr. Kwan Ho Tang
Dr. Polly Chen
Mr. Pak Shing Kwan

Department of Surgery, HKU
Prof. Chung Mau Lo
Dr. Kwan MAN
Department of Pathology, HKU
Dr. Kwok Wah Chan

Thank You
Questions or Comments
Hong Kong

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